HPLC, or High Performance Liquid Chromatography, is a powerful and widely used analytical technique essential for drug development, quality control, and various research applications. It enables scientists and analysts to separate, identify, and quantify individual components within complex mixtures with high precision and accuracy. HPLC plays a crucial role in pharmaceutical laboratories, chemical industries, academic research, and biotechnology, where reliable and reproducible results are vital.

This easy-to-understand guide explains how HPLC works, including its core components such as